Format

Send to

Choose Destination
Eur J Clin Microbiol Infect Dis. 1995 Aug;14(8):713-6.

Use of a new oligonucleotide probe for detection of colonization factor antigen III gene in enterotoxigenic Escherichia coli.

Author information

1
Department of Bacterial Infections, Osaka University, Japan.

Abstract

An alkaline phosphatase-labeled 30-mer oligonucleotide probe was designed to detect the gene for pilus colonization factor antigen III (CFA/III) of the human type of enterotoxigenic Escherichia coli (ETEC). The CFA/III probe was used to identify CFA/III-producing ETEC among 303 Escherichia coli obtained from subjects with traveler's diarrhea. Six isolates positive for the CFA/III gene were found. This result was confirmed immunologically by using a specific monoclonal antibody developed against CFA/III. These six isolates, isolated from travelers returning from India, Pakistan and China, were all positive for the gene of heat-labile enterotoxin and possessed an identical serotype (025:H-).

PMID:
8565993
DOI:
10.1007/bf01690882
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center