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Am J Surg. 1996 Jan;171(1):41-6.

Prognostic value of TP53 and K-ras-2 mutational analysis in stage III carcinoma of the colon.

Author information

1
Department of Surgery, Brown University School of Medicine, Providence, Rhode Island, USA.

Abstract

BACKGROUND:

Genetic mutations involving oncogenes and tumor-suppressor genes occur in carcinogenesis, and may affect biologic behavior of neoplasms. In this study, we analyzed the prognostic value of mutational analysis in colon carcinoma.

PATIENTS AND METHODS:

Archival pathology specimens from 70 consecutive patients, resected for stage III colon carcinoma, were analyzed for point mutations by amplification and direct sequencing of exons from the K-ras-2 and the TP53 genes (topographic genotyping). Mutations were compared with adverse histopathologic features (poor differentiation, vascular and lymphatic invasion, mucin production) as prognostic markers.

RESULTS:

Five-year survival was 75% in patients with nonmutated lesions, significantly lower (21%) with TP53 mutations (P = 0.01), and intermediate with K-ras-2 only (45%) or both K-ras-2 and TP53 mutations (36%). A TP53 mutation carried the highest relative risk of death (2.39; 95% confidence interval, 1.29 to 4.42; P = 0.006). There was an additive effect on the risk of death between TP53 mutations and adverse histopathologic features.

CONCLUSIONS:

The information derived from mutational analysis is creating new prognostic variables that may play a role in the choice of therapy for colorectal carcinoma.

PMID:
8554149
DOI:
10.1016/S0002-9610(99)80071-3
[Indexed for MEDLINE]

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