Send to

Choose Destination
Nat Struct Biol. 1996 Jan;3(1):32-7.

A novel form of the DNA double helix imposed on the TATA-box by the TATA-binding protein.

Author information

Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.


The structure of the TATA-box bound to the TATA-binding protein revealed a new and unexpected deformation of the double helix leading to a sharp change in the DNA trajectory. Here we show that the deformation imposed upon the TATA-box represents a novel form of the double helix--named TA-DNA--which differs from A-DNA by a single conformational parameter, namely the rotation around the glycosidic bond. This rotation causes a 50 degrees inclination of the base pairs in the TATA-box which in turn results in abrupt change in the trajectory of the flanking B-DNA segments. The observation that the TATA sequence can assume an A-DNA conformation coupled to the simplicity of the transition from A-DNA to TA-DNA may be the reason for the presence of the TATA sequence in a wide range of promoters.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center