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Neurobiol Aging. 1995 Sep-Oct;16(5):755-64.

Soluble multimeric Alzheimer beta(1-40) pre-amyloid complexes in dilute solution.

Author information

1
Department of Neurodegenerative Diseases, Parke-Davis Pharmaceutical Research Division of the Warner-Lambert Company, Ann Arbor, MI 48106-1047, USA.

Abstract

Aqueous solutions of beta(1-40) peptide spontaneously associate to form pentameric/hexameric complexes that can be demonstrated by SDS-PAGE following treatment with glutaraldehyde and borohydride reduction. Under amyloidogenic conditions of pH and high peptide concentration these aggregates can further associate to form pentameric/hexameric complexes that can be demonstrated by SDS-PAGE following treatment with glutaraldehyde and borohydride reduction. Under amyloidogenic conditions of pH and high peptide concentration these aggregates can further associate to form sedimentable and filterable structures with beta-sheet amyloid characteristics of Thioflavine T fluorescence. The presence of such preamyloid structures at low peptide concentration suggests a mechanism by which amyloid plaques can accrete additional material by a cooperative rather than monomeric growth. The existence of a monomer<==>multimer equilibrium may partly explain the divergence of biological consequences with respect to neurotoxicity.

PMID:
8532108
[Indexed for MEDLINE]

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