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Gene. 1995 Dec 1;166(1):77-82.

Cloning and comparison of mercury- and organomercurial-resistance determinants from a Pseudomonas stutzeri plasmid.

Author information

1
Dipartimento di Genetica e di Biologia dei Microrganismi, Milano, Italy.

Abstract

Plasmid pPB confers broad-spectrum mercury resistance (HgR) to a Pseudomonas stutzeri strain. Two pPB regions, separated by 25-30 kb and sharing homology with Tn501 mer (Hg detoxification) genes, were cloned separately and each was shown to carry a cluster of functional and independently regulated mer genes. One of the two gene clusters conferred resistance only to inorganic mercury, and had a structure identical to the classical model of narrow-spectrum mer operons. In the other cluster a novel merB gene, not homologous to the other known merB, but with the same function, was mapped upstream from merA, interposed between an organomercurial-responsive regulatory element and transport genes. Evidence suggests that merB and the other structural mer genes might be transcribed from two distinct promoters. The presence of two inverted repeat-like elements, identical to those of Tn5053, upstream from merR suggests that the pPB broad-spectrum-gene cluster could be part of a transposon-like element.

PMID:
8529897
DOI:
10.1016/0378-1119(95)00546-4
[Indexed for MEDLINE]

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