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Gene. 1995 Dec 1;166(1):181-2.

Color selection with a hygromycin-resistance-based Escherichia coli-mycobacterial shuttle vector.

Author information

1
Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.

Abstract

Hygromycin-resistance (HyR)-based Escherichia coli-mycobacterial shuttle plasmids have high efficiencies of transformation and a broad mycobacterial host range. We have introduced a lacZ alpha (encoding the alpha-polypeptide fragment of beta-galactosidase (beta Gal))-multiple cloning site cassette into a HyR-based shuttle vector to generate a plasmid with nine unique cloning sites and the added feature of beta Gal color selection in appropriate E. coli host strains.

PMID:
8529888
DOI:
10.1016/0378-1119(95)00597-x
[Indexed for MEDLINE]

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