Format

Send to

Choose Destination
Cell. 1995 Dec 1;83(5):783-91.

Identification of double Holliday junctions as intermediates in meiotic recombination.

Author information

1
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

Abstract

During meiosis, branched DNA molecules containing information from both parental chromosomes occur in vivo at loci where meiosis-specific double-stranded breaks occur. We demonstrate here that these joint molecules are recombination intermediates: they contain single strands that have undergone exchange of information. Moreover, these joint molecules are resolved into both parental and recombinant duplexes when treated in vitro with Holliday junction-resolving endonucleases RuvC or T4 endo VII. Taken together with previous observations, these results strongly suggest that joint molecules are double Holliday junctions.

PMID:
8521495
DOI:
10.1016/0092-8674(95)90191-4
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center