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Cell. 1995 Dec 1;83(5):783-91.

Identification of double Holliday junctions as intermediates in meiotic recombination.

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Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.


During meiosis, branched DNA molecules containing information from both parental chromosomes occur in vivo at loci where meiosis-specific double-stranded breaks occur. We demonstrate here that these joint molecules are recombination intermediates: they contain single strands that have undergone exchange of information. Moreover, these joint molecules are resolved into both parental and recombinant duplexes when treated in vitro with Holliday junction-resolving endonucleases RuvC or T4 endo VII. Taken together with previous observations, these results strongly suggest that joint molecules are double Holliday junctions.

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