Heterogeneity within the first constant segment of the major outer membrane protein gene in Chlamydia trachomatis serovar D/Da distinguishes 2 lineages

C R Acad Sci III. 1995 Sep;318(9):943-9.

Abstract

Restriction fragment length polymorphism of the major outer membrane protein gene (ompl) was studied in 73 epidemiologically unrelated Chlamydia trachomatis serovar D (n = 64) and Da (n = 9) strains isolated between 1983 and 1991 in various european countries from patients suffering from sexually transmitted diseases, as well as 3 reference strains D/IC-Cal-8 (USA), D/UW3 (UK) and Da/TW-448 (Taiwan). Among these strains, 5 different genotypic groups were distinguished: 3 for the serovar D strains and 2 for the serovar Da strains. Comparisons of the complete ompl nucleotide sequence of 1 member of each group revealed 2 distinct lineages, independently of the D/Da classification, presumably as the result of an intragenic DNA recombination event within the first constant segment. The observed ompl genetic diversity of serovar D/Da strains in regions involved in T- and B-cell responses might add another level of complexity to the design of a subunit vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Outer Membrane Proteins / genetics*
  • Base Sequence
  • Cell Line
  • Chlamydia trachomatis / classification
  • Chlamydia trachomatis / genetics*
  • Genetic Heterogeneity
  • In Vitro Techniques
  • Molecular Sequence Data

Substances

  • Bacterial Outer Membrane Proteins

Associated data

  • GENBANK/X77365