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Mol Biotechnol. 1995 Aug;4(1):13-5.

Precise large deletions by the PCR-based overlap extension method.

Author information

1
Department of Microbiology, University of Tennessee, Knoxville 37996-0845, USA.

Abstract

The authors describe an efficient method for generating large deletions (> 200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.

PMID:
8521036
[Indexed for MEDLINE]

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