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Obstet Gynecol. 1993 Jul;82(1):29-36.

Production of fibrogenic cytokines by interleukin-2-treated peripheral blood leukocytes: expression of transforming growth factor-beta and platelet-derived growth factor B chain genes.

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Department of Cell Biology, Neurobiology, and Anatomy, Loyola University Stritch School of Medicine, Maywood, Illinois.



To assess the production of fibrogenic cytokines by interleukin-2 (IL-2)-stimulated peripheral blood leukocytes and to examine their ability to stimulate the production of connective tissue.


Culture medium from human peripheral blood leukocytes incubated with or without IL-2 was tested for induction of fibroblast proliferation, collagen synthesis, and expression of cytokine genes.


Supernatants from IL-2-treated peripheral blood leukocytes induced six times more fibroblast proliferation than medium from leukocytes cultured without IL-2. The expression of type I procollagen and fibronectin messenger RNAs was increased in human fibroblasts in response to leukocyte supernatants. Unstimulated leukocytes expressed minimal levels of transforming growth factor-beta or platelet-derived growth factor B chain messenger RNAs, but could be greatly enhanced by IL-2 treatment.


Mediators that induce connective tissue production are secreted by IL-2-treated peripheral blood leukocytes. These cytokines may be responsible, in part, for the stimulation of abdominal adhesions in patients receiving intraperitoneal immunotherapy.

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