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FEBS Lett. 1993 Jun 1;323(3):198-202.

The cis/trans interconversion of the calcium regulating hormone calcitonin is catalyzed by cyclophilin.

Author information

1
Max-Planck-Gesellschaft, Arbeitsgruppe Enzymologie der Peptidbindung, Halle/Saale, Germany.

Abstract

The cytosolic peptidyl-prolyl cis/trans isomerase cyclophilin from pig kidney can accelerate catalytically the cis/trans isomerization of prolyl peptide bonds. One- and two-dimensional 1H NMR spectroscopy was used to prove that the polypeptide hormone calcitonin is a substrate for cyclophilin. Isomerization of only one of the two prolyl peptide bonds is catalyzed significantly. The efficiency of catalysis was calculated by lineshape analysis and NOESY spectroscopy. Cyclosporin A completely blocks the effect of the enzyme on the conformational dynamics of the polypeptide.

PMID:
8500610
DOI:
10.1016/0014-5793(93)81338-z
[Indexed for MEDLINE]
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