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Fertil Steril. 1993 Jun;59(6):1196-201.

Effect of tumor necrosis factor-alpha on adhesion of human endometrial stromal cells to peritoneal mesothelial cells: an in vitro system.

Author information

1
Department of Obstetrics and Gynecology, University of Oklahoma Health Sciences Center, Oklahoma City 73190.

Abstract

OBJECTIVE:

To assess the effects of tumor necrosis factor-alpha (TNF-alpha) on adhesion of endometrial stromal cells to peritoneal mesothelial cells, a possible step leading to endometriosis.

DESIGN:

Analysis of cell adhesion in vitro.

SETTING:

University research laboratory.

PATIENTS:

Peritoneal fluid and biopsies were obtained from volunteers with regular cycles without endometriosis or endometrial pathology.

INTERVENTIONS:

Tumor necrosis factor-alpha (0.1 to 1,000 U/mL) was added to nutrient media with the following supplements: 10% fetal calf serum, epidermal growth factor 20 ng/mL, and gentamicin 10 ng/mL.

MAIN OUTCOME MEASURES:

Radioactivity of chromium-51 was used as a reflection of adherent stromal cells.

RESULTS:

The adherence of endometrial stromal cells to mesothelial cells was significantly increased by pretreatment of mesothelial cells with TNF-alpha.

CONCLUSIONS:

Tumor necrosis factor-alpha may play a facilitory role in the development of endometriosis.

PMID:
8495765
DOI:
10.1016/s0015-0282(16)55976-7
[Indexed for MEDLINE]

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