Modified immunological status of anti-IL-10 treated mice

Cell Immunol. 1993 May;148(2):371-84. doi: 10.1006/cimm.1993.1119.

Abstract

We have shown that continuous treatment of mice from birth to adulthood with neutralizing anti-IL-10 antibodies leads to specific depletion of Ly1 B cells, while conventional B cells remain normal in terms of number, phenotype, and function. Extending our characterization of these animals, we show here that anti-IL-10 treated mice can be distinguished from untreated or isotype control treated mice by several other criteria. Anti-IL-10 treated mice contained substantially elevated levels of circulating TNF-alpha, and in many cases circulating IL-6, and were profoundly susceptible to death by LPS-induced shock, a monokine mediated inflammatory reaction. Analysis of serum immunoglobulin levels in anti-IL-10 treated mice revealed a decrease in serum IgA levels to accompany the previously reported reduction in serum IgM, plus a striking increase in IgG2a and IgG2b levels. Further investigation of the Ly1 B cell depletion of anti-IL-10 treated mice revealed that this effect was transient as evidenced by the return of Ly1 B cells in normal numbers 8 weeks after anti-IL-10 treatment was discontinued. The Ly1 B cell depletion that occurred during anti-IL-10 treatment was found to be compensated by an increase in peritoneal T cells and granulocytes. Finally, while anti-IL-10 treated mice were unable to produce antibodies to phosphorylcholine and alpha 1,3-dextran, they developed normal antibody responses following intraperitoneal injections of TNP-Ficoll, suggesting the existence of subcategories within the family of thymus independent type II polysaccharide antigens. These data are discussed within the context of their implications for the roles of IL-10 and Ly1 B cells in the immune system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibody Formation*
  • Antigens, T-Independent / immunology
  • B-Lymphocyte Subsets / immunology*
  • Cytokines / metabolism*
  • Granulocytes / cytology
  • Immunoglobulins / blood*
  • Interleukin-10 / physiology*
  • Leukocyte Count
  • Lymphocyte Depletion
  • Mice
  • Mice, Inbred BALB C
  • Peritoneal Cavity / cytology
  • T-Lymphocytes / cytology
  • Time Factors

Substances

  • Antibodies, Monoclonal
  • Antigens, T-Independent
  • Cytokines
  • Immunoglobulins
  • Interleukin-10