Humoral immune response of humans to lipooligosaccharide and outer membrane proteins of Haemophilus ducreyi

J Infect Dis. 1993 May;167(5):1206-10. doi: 10.1093/infdis/167.5.1206.

Abstract

The humoral immune response to purified lipooligosaccharide (LOS) and outer membrane proteins (OMP) of Haemophilus ducreyi was evaluated. Sera from chancroid-endemic (Uganda, Kenya) and -nonendemic (Canada) countries were tested by an ELISA. The response to OMPs was cross-reactive with other Haemophilus species, and elevated levels of antibody were detected in patients that did not have chancroid. The LOS component stimulated an H. ducreyi-specific immune response that was detected only in patients with chancroid. The sensitivity of the LOS ELISA was 96% (95% confidence interval, 89.9%-100%) and the specificity was 97% (95% confidence interval, 95.8%-98.2%). Thus, the anti-H. ducreyi LOS immune response is a significant diagnostic and epidemiologic indicator.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antibodies, Bacterial / immunology*
  • Bacterial Outer Membrane Proteins / immunology*
  • Chancroid / immunology*
  • Child
  • Child, Preschool
  • Female
  • Haemophilus ducreyi / immunology*
  • Humans
  • Infant
  • Lipopolysaccharides / immunology*
  • Male

Substances

  • Antibodies, Bacterial
  • Bacterial Outer Membrane Proteins
  • Lipopolysaccharides