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Methods Enzymol. 1993;217:483-509.

Optimizing the biolistic process for different biological applications.

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Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva 14456.


The biolistic process is still rapidly evolving. We do not anticipate further major improvements in biolistic apparatus. There will probably still be further major improvements in particles, DNA coating, and vectors, as well as significant further advances in understanding of biological determinants of cell penetration and survival. The technology has currently reached the point at which it can be readily and reliably used for a wide range of applications. Given the information presented in this chapter, new applications can be optimized fairly readily.

[Indexed for MEDLINE]

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