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Anal Biochem. 1993 Mar;209(2):274-7.

Synthesis of 3,4-cis-[3H]leucocyanidin and enzymatic reduction to catechin.

Author information

1
CSIRO Division of Plant Industry, Canberra, ACT, Australia.

Abstract

A novel method is presented for the synthesis and purification of (+)-2,3-trans-3,4-cis-[4-3H]leucocyanidin. Soluble enzyme extracts from developing barley grains and leaves of the forage legume Onobrychis viciifolia (sainfoin) catalyzed the NAD(P)H-dependent reduction of (+)-2,3-trans-3,4-cis-[4-3H]leucocyanidin to (+)-[4-3H]catechin. NADPH was the preferred substrate. With extracts of barley the rate of reaction with 1 mM NADH was 20% of the rate found with NADPH. With extracts from both tissues there was a broad pH optimum around pH 6.6.

PMID:
8470799
DOI:
10.1006/abio.1993.1119
[Indexed for MEDLINE]

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