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Arthritis Rheum. 1993 Apr;36(4):552-60.

Nucleosome-specific antibody from an autoimmune MRL/Mp-lpr/lpr mouse.

Author information

1
Garden State Cancer Center, Newark, New Jersey.

Abstract

OBJECTIVE:

To characterize the binding properties and variable-region sequences of LG4-1, a monoclonal antibody from an autoimmune MRL/Mp-lpr/lpr mouse that reacts specifically with nucleosome core particles and represents a new antinuclear antibody specificity.

METHODS:

The reactivity of the antibody against various nuclear substrates was determined using an enzymatic immunoassay, and the variable-region genes were sequenced from messenger RNA, using the dideoxy chain termination method.

RESULTS:

LG4-1 was found to react with nucleosome core particles but not with individual histones and DNA, or with various histone-histone and histone-DNA complexes. It was demonstrated that this antibody is encoded by a combination of variable-region genes and gene segments that have undergone few somatic mutations.

CONCLUSION:

The nucleosome core particle expresses a unique conformational autoepitope(s) resulting from the ordered association of histones and DNA.

PMID:
8457229
DOI:
10.1002/art.1780360417
[Indexed for MEDLINE]

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