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Nucleic Acids Res. 1993 Feb 25;21(4):969-75.

Activity of yeast FLP recombinase in maize and rice protoplasts.

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1
Department of Botany and Plant Pathology, Purdue University, West Lafayette, IA 47907.

Abstract

We have demonstrated that a yeast FLP/FRT site-specific recombination system functions in maize and rice protoplasts. FLP recombinase activity was monitored by reactivation of beta-glucuronidase (GUS) expression from vectors containing the gusA gene inactivated by insertion of two FRTs (FLP recombination targets) and a 1.31 kb DNA fragment. The stimulation of GUS activity in protoplasts cotransformed with vectors containing FRT inactivated gusA gene and a chimeric FLP gene depended on both the expression of the FLP recombinase and the presence and structure of the FRT sites. The FLP enzyme could mediate inter- and intramolecular recombination in plant protoplasts. These results provide evidence that a yeast recombination system can function efficiently in plant cells, and that its performance can be manipulated by structural modification of the FRT sites.

PMID:
8451196
PMCID:
PMC309231
[Indexed for MEDLINE]
Free PMC Article
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