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Cancer. 1993 Mar 15;71(6):1960-3.

Isolation of Inoue-Melnick virus from colorectal carcinoma tissues.

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1
Department of Biological Resources, Roswell Park Cancer Institute, Buffalo, New York 14263.

Abstract

BACKGROUND:

Isolation of Inoue-Melnick virus (IMV) Type 1 from tumor tissues of patients with colorectal carcinoma in Japan was reported. A high prevalence of IMV antibodies has been found among patients with colorectal carcinoma in Buffalo, New York.

METHODS:

IMV was isolated from homogenates of colorectal carcinoma and other tissues, inoculated into MRC-5 human fibroblast cultures. IMV-specific cytopathic effects were detected and titrated following 7 days of incubation in a roller-tube apparatus.

RESULTS:

IMV was isolated from all nine (100%) colorectal carcinoma tissues tested and all four (100%) normal parts of colon mucosa tissues obtained from patients with cancer. Biopsy specimens of normal colon mucosa from 10 patients without colon carcinoma were negative for IMV (0%). Average infectious titers of IMV in these tissue homogenates were 4.41 for tumor tissue and 2.55 log10 tissue culture infectious dose by 50% (TCID50)/0.1 ml for colon mucosa, respectively. Most of the isolates were the IMV intermediate type.

CONCLUSIONS:

Our results confirmed the production of infectious IMV in colorectal carcinoma tissues, as reported recently in Japan. The role of IMV in colorectal carcinogenesis is currently unknown.

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