Format

Send to

Choose Destination
See comment in PubMed Commons below
Arch Biochem Biophys. 1993 Feb 1;300(2):756-60.

Quantitative in vitro assay for human immunodeficiency virus deoxyribonucleic acid integration.

Author information

1
Laboratoire de Pharmacologie Mol├ęculaire, CNRS URA 147, INSERM U 140, Institut Gustave Roussy, Villejuif, France.

Abstract

An obligatory step of retroviral growth is the integration of a DNA copy of the viral RNA into the genomic DNA of the host. Recombinant human immunodeficiency virus type I (HIV-1) integrase (IN) expressed in Escherichia coli efficiently catalyzes the overall in vitro integration reaction, namely, the processing of the long terminal repeat (LTR) ends and the strand transfer reaction. Using the 3' end of synthetic oligonucleotides which match the termini of HIV-1 LTRs as substrate and supercoiled pSP65 DNA as the target, we describe an assay that is suitable for the enzymatic analysis of the integration and for testing candidate inhibitors of HIV IN protein.

PMID:
8434953
DOI:
10.1006/abbi.1993.1105
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center