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Biotechniques. 1993 Feb;14(2):225-6.

Transformation of Helicobacter pylori by electroporation.

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Department of Microbiology and Immunology, Stanford University, CA 94305.


A highly efficient method of introducing DNA into an important human pathogen is reported here. Electroporation-mediated transformation of a laboratory-passaged isolate of Helicobacter pylori was successfully used to establish genetic mutants at a transformation frequency of > 10(5)/micrograms DNA. This method should be widely applicable to all isolates of H. pylori and may eliminate the variability reported when natural transformation was used on fresh clinical isolates.

[Indexed for MEDLINE]

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