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Biotechniques. 1993 Feb;14(2):222-4.

SP6 RNA polymerase containing vaccinia virus for rapid expression of cloned genes in tissue culture.

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Natl. Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892.


A hybrid transient expression system, in which tissue culture cells are infected with a recombinant vaccinia virus encoding bacteriophage DNA-dependent RNA polymerase and transfected with a plasmid containing a cloned gene behind the bacteriophage promoter, allows rapid high-level expression in nearly 100% of the cells. In order to extend this system to clones from libraries containing SP6 promoters, a new vaccinia virus was constructed encoding bacteriophage SP6 RNA polymerase.

[Indexed for MEDLINE]

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