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Crit Care Med. 1993 Feb;21(2):196-202.

Human sepsis increases lymphocyte intracellular calcium.

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1
Department of Anesthesia, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157-1009.

Abstract

OBJECTIVE:

To determine whether free intracellular calcium is increased during human bacterial sepsis.

DESIGN:

Prospective controlled study of lymphocyte free intracellular calcium concentrations from patients with sepsis compared with critically ill nonseptic patients and healthy subjects.

SETTING:

A large multidisciplinary ICU of a university hospital.

PATIENTS:

Eleven patients with sepsis, six patients after cardiac surgery, six patients with head injury, and 22 healthy control subjects.

INTERVENTIONS:

Blood samples obtained for lymphocyte isolation and measurement of free intracellular calcium concentrations.

MEASUREMENTS:

Lymphocytes were isolated using Ficoll-paque centrifugation and free intracellular calcium concentrations were measured using the fluorescent dye fura-2. We also evaluated the effect of septic serum, endotoxin, tumor necrosis factor (TNF), and lysophosphatidylcholine on lymphocyte free intracellular calcium concentrations.

MAIN RESULTS:

Mean (+/- SEM) lymphocyte free intracellular calcium concentrations were significantly (p < .05) higher in the septic patients (176 +/- 12 nM) compared with cardiac surgical (112 +/- 9 nM), head-injured (110 +/- 11 nM), or healthy control patients (112 +/- 5 nM). Endotoxin (0.1 and 1.0 mg/mL) and TNF (10 and 100 ng/mL) did not alter lymphocyte free intracellular calcium values. Lysophosphatidylcholine (100 and 200 microM) significantly increased lymphocyte free intracellular calcium in a dose-dependent manner. Septic serum had no effect on resting lymphocyte free intracellular calcium concentrations but potentiated the free intracellular calcium response to lysophosphatidylcholine.

CONCLUSIONS:

Lymphocyte intracellular calcium homeostasis is altered during human sepsis. In addition, circulating factors present in septic serum are capable of altering cellular calcium handling.

[Indexed for MEDLINE]

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