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Gastroenterology. 1993 Feb;104(2):467-74.

The effect of enterohemorrhagic Escherichia coli O157:H7 on intestinal structure and solute transport in rabbits.

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1
Department of Pediatrics and Child Health, Children's Hospital, Camperdown, Australia.

Abstract

BACKGROUND:

The effect of enterohemorrhagic Escherichia coli O157:H7 infection on intestinal morphology and solute transport was examined.

METHODS:

New Zealand white rabbits, aged 10 days, were infected with E. coli strain EDL933 (O157:H7 containing the 60-megadalton plasmid-encoding adhesion factors VT1 and VT2) and compared with controls. Small and large intestinal histology and solute transport were studied 5 days after inoculation. Ion transport in the distal colon was also examined in animals infected with different strains encoding a combination of pathogenic factors.

RESULTS:

Infection with EDL933 induced diarrhea and mucosal disease in the colon, inhibited colonic Na+ absorption, and stimulated of Cl- secretion, but had no impact on the small intestine. Infection with strains A7785-C3A (O157:H7, plasmid-, VT1+, VT2+) and 85-170 (O157:H7, plasmid+, VT-) induced similar transport changes to EDL933. C600/1 (E. coli K-12, plasmid+, VT1+) decreased Na+ and Cl- absorption only.

CONCLUSIONS:

Abnormalities of colonic structure and ion transport could account for diarrhea production, but pathogenic factors other than the 60-megadalton plasmid-encoding adhesion factor and verotoxins appear to be involved in enterohemorrhagic E. coli infection.

PMID:
8425689
DOI:
10.1016/0016-5085(93)90415-9
[Indexed for MEDLINE]

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