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Gene. 1993 Jan 15;123(1):93-7.

Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824.

Author information

1
F.A. Janssens Laboratorium voor Genetica, K.V. Leuven, Heverlee, Belgium.

Abstract

The nucleotide sequence of three open reading frames in the acetone-production locus of Clostridium acetobutylicum ATCC824 has been established. The three gene products, corresponding to acetoacetate decarboxylase (EC 4.1.1.4) and both subunits of the acetoacetyl-CoA:acetate/butyrate:CoA transferase (EC 2.8.3.9) are transcribed in two convergently arranged operons. The intervening DNA region separating the two transcripts is characterized by an inverted repeat which appears capable of forming a stem-loop structure functioning as a Rho-independent transcription terminator in both directions.

PMID:
8423010
DOI:
10.1016/0378-1119(93)90545-e
[Indexed for MEDLINE]

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