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Genes Dev. 1993 Jan;7(1):42-54.

Sex-specific transcriptional regulation by the male and female doublesex proteins of Drosophila.

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  • 1Department of Biochemistry, Brandeis University, Waltham Massachusetts 02254-9110.

Abstract

The somatic sexual phenotype of Drosophila is regulated by the sexual differentiation pathway. Male (DSXM) and female (DSXF) proteins encoded by doublesex (dsx), a gene at the end of this pathway, bind to three sites within a 127-bp enhancer that directs sex- and tissue-specific transcription of Yolk protein genes. We describe mutagenesis of these binding sites and the resulting effects on DSXM and DSXF binding in vitro and on gene regulation in wild-type and dsx mutant flies. The results demonstrate that DSXM represses and DSXF activates transcription from the two strongest binding sites. Thus, the pathway regulates sex-specific transcription through the male and female dsx proteins that act directly on the target gene, but with opposite effects.

PMID:
8422987
[PubMed - indexed for MEDLINE]
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