Interference of carbamylated and acetylated hemoglobins in assays of glycohemoglobin by HPLC, electrophoresis, affinity chromatography, and enzyme immunoassay

Clin Chem. 1993 Jan;39(1):138-42.

Abstract

In vitro-synthesized carbamylated and acetylated hemoglobins interfered in assays of glycohemoglobin by HPLC and electrophoresis but had no effects on results obtained by affinity chromatography and enzyme immunoassay. Correlations between long-term serum urea concentrations and glycohemoglobin percentages revealed that, in vivo, carbamylated hemoglobin equivalent to 0.063% of total hemoglobin is formed for every 1 mmol/L of serum urea. The use of acetylsalicylate, either chronically in small doses (200-300 mg/day) or for 1 week at 2000 mg/day, did not cause significant interference from acetylhemoglobin, formed in vivo. We conclude that interference from carbamylated hemoglobin explains only a small part of existing discrepancies between results of glycohemoglobin assays in current use. The interfering effect of acetylhemoglobin formed in vivo with acetyl-CoA as substrate is as yet unknown.

MeSH terms

  • Acetylation
  • Adult
  • Aged
  • Aged, 80 and over
  • Aspirin / blood
  • Aspirin / therapeutic use
  • Blood Glucose / analysis
  • Chromatography*
  • Chromatography, Affinity
  • Chromatography, High Pressure Liquid
  • Electrophoresis*
  • Glycated Hemoglobin / analysis*
  • Hemoglobin A / analogs & derivatives*
  • Hemoglobins*
  • Humans
  • Immunoenzyme Techniques*
  • Middle Aged
  • Quality Control
  • Urea / blood

Substances

  • Blood Glucose
  • Glycated Hemoglobin A
  • Hemoglobins
  • acetylated hemoglobin
  • hemoglobin A, carbamylated
  • Urea
  • Hemoglobin A
  • Aspirin