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Nature. 1993 Oct 14;365(6447):671-3.

Quantitative detection of HIV-1 drug resistance mutations by automated DNA sequencing.

Author information

1
Antiviral Therapeutic Research Unit, Wellcome Foundation Limited, Beckenham, Kent, UK.

Abstract

A comparison has been made between manual and automated DNA sequencing procedures to evaluate the ability to distinguish mixtures of wild-type and mutant sequences. Quantitative detection of such mixtures of HIV-1 drug resistance mutations was best achieved using an automated system that uses fluorescent-labelled sequencing primers. This procedure has a wide range of applications in clinical research, including heterozygote analysis. Software that automatically reports mixed-base positions is presented.

PMID:
8413632
DOI:
10.1038/365671a0
[Indexed for MEDLINE]

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