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Nature. 1993 Oct 14;365(6447):661-3.

The GTP-binding protein Ran/TC4 is required for protein import into the nucleus.

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Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021.


Two cytosolic fractions (A and B) from Xenopus oocytes are sufficient to support protein import into the nuclei of digitonin-permeabilized cells. Fraction A recognizes the nuclear localization sequence (NLS) and binds the import substrate to the nuclear envelope, whereas fraction B mediates the subsequent passage of the bound substrate into the nucleus. Here we report that two interacting components are required for full fraction-B activity, purify one of these components to homogeneity, and show that it is the highly abundant GTP-binding protein Ran (Ras-related nuclear protein)/TC4.

[Indexed for MEDLINE]

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