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Microb Pathog. 1993 Jul;15(1):77-82.

Polymerase chain reaction amplification, cloning and sequencing of variant Escherichia coli Shiga-like toxin type II operons.

Author information

1
Department of Microbiology, Adelaide Children's Hospital, South Australia.

Abstract

The polymerase chain reaction (PCR) was used to amplify approximately 1.5 kb segments of DNA containing complete Shiga-like toxin type II operons from Escherichia coli serotypes OX3:H21 and O111:H-. These fragments were cloned and DNA sequence analysis identified further variations compared with published SLT-II sequences.

PMID:
8412629
DOI:
10.1006/mpat.1993.1058
[Indexed for MEDLINE]

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