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Microb Pathog. 1993 Jun;14(6):495-500.

IFN-gamma produced in vivo during the first two days is critical for resolution of murine Leishmania major infections.

Author information

1
Department of Cellular Immunology, Walter Reed Army Institute of Research, Washington, DC 20307-5100.

Abstract

Resolution of murine infection with Leishmania major is dependent upon the presence of IFN-gamma during the first week of infection. To more precisely determine the period during which IFN-gamma is critical, we infected footpads of resistant C3H/HeN mice with amastigotes of L. major and treated these mice with neutralizing monoclonal Ab (MAb) specific for IFN-gamma on successive days. Mice treated with anti-IFN-gamma MAb on or before day 2 had significantly enlarged lesions, and increased parasites in lesions, compared with mice treated with an isotype control MAb. In contrast, mice treated with anti-IFN-gamma MAb on day 3 or later resolved their lesions and had no parasites at the inoculation site. Related experiments obtained with a neutralizing MAb specific for TNF-alpha demonstrated the critical role TNF-alpha plays in resolution of Leishmania infection. Thus, IFN-gamma and TNF-alpha were both critical for resolution of infection with L. major, with IFN-gamma's role limited to the first 2 days.

PMID:
8412622
DOI:
10.1006/mpat.1993.1049
[Indexed for MEDLINE]

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