Format

Send to

Choose Destination
FEBS Lett. 1993 Oct 11;332(1-2):74-80.

Cloning, sequence and expression of the gene encoding the malolactic enzyme from Lactococcus lactis.

Author information

1
Laboratoire de Microbiologie et Technologie des Fermentations, Institut des Produits de la Vigne, INRA, Montpellier, France.

Abstract

Many lactic acid bacteria can carry out malolactic fermentation. This secondary fermentation is mediated by the NAD- and Mn(2+)-dependent malolactic enzyme, which catalyses the decarboxylation of L-malate to L-lactate. The gene we call mleS, coding for malolactic enzyme, was isolated from Lactococcus lactis. The mleS gene consists of one open reading frame capable of coding for a protein with a calculated molecular mass of 59 kDa. The amino acid sequence of the predicted MleS gene product is homologous to the sequences of different malic enzymes. Bacterial and yeast cells expressing the malolactic gene convert L-malate to L-lactate.

PMID:
8405453
DOI:
10.1016/0014-5793(93)80488-g
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center