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FEBS Lett. 1993 Jun 21;324(3):349-52.

Receptor-binding capability of pancreatic phospholipase A2 is separable from its enzymatic activity.

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1
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.

Abstract

Mammalian pancreatic phospholipase A2 (PLA2-I) has its specific receptor through which PLA2-I induces a variety of biological responses. In this study, a fundamental relationship between the enzymatic and the receptor-binding activities of PLA2-I was investigated. The specific binding of PLA2-I to the receptor was found to be independent of Ca2+ which is requisite for the PLA2 activity. On the basis of this observation, we designed and produced mutant PLA2-Is without Ca(2+)-binding abilities in order to demonstrate that the structural requirement for the enzymatic activity of PLA2-I is not identical with that for its receptor-binding reaction. These mutant PLA2-Is lost almost all enzymatic activity through a disturbance at the Ca(2+)-binding site, as expected, but still retained a substantial affinity to the receptor, allowing us to conclude that the receptor-binding reaction of PLA2-I is separable from its catalytic action.

PMID:
8405380
DOI:
10.1016/0014-5793(93)80149-o
[Indexed for MEDLINE]
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