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Biol Mass Spectrom. 1993 Sep;22(9):544-50.

Reproducibility and quantitation of matrix-assisted laser desorption ionization mass spectrometry: effects of nitrocellulose on peptide ion yields.

Author information

1
Texas A & M University, Department of Chemistry, College Station 77840.

Abstract

We have observed that using nitrocellulose in sample preparation for matrix-assisted laser desorption ionization (MALDI) time-of-flight mass spectrometry increases the yield of peptide [M+H]+ ions. Addition of nitrocellulose also provides improvement in sample-to-sample reproducibility of MALDI ion yield and improves the precision of peptide quantitation. Mass spectrometry and optical microscopy results suggest that the nitrocellulose modifies the crystallization of matrix/analyte solution to allow more even coverage over the sample surface.

PMID:
8399403
DOI:
10.1002/bms.1200220908
[Indexed for MEDLINE]

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