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J Antimicrob Chemother. 1993 Jun;31 Suppl E:1-4.

In-vitro concentration of azithromycin in human phagocytic cells.

Author information

1
Institut Pasteur de Lyon, France.

Abstract

The in-vitro intraphagocytic uptake and retention of azithromycin in both human polymorphonuclear leucocytes (PMN) and alveolar macrophages was measured by an improved high-performance liquid chromatography (HPLC) method that was approximately three-fold more sensitive than previous methods. Azithromycin was accumulated in PMN and alveolar macrophages (about 300-fold), with maximum uptake being obtained after incubation for 60 min. Azithromycin was eliminated only partially from the cells during the washing process, and was released slowly during re-incubation of phagocytic cells in antibiotic-free medium. This intracellular retention distinguishes azithromycin from most of the macrolides and quinolones which, in spite of high I/E ratios, are released rapidly from cells.

PMID:
8396080
DOI:
10.1093/jac/31.suppl_e.1
[Indexed for MEDLINE]

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