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Neuron. 1993 Apr;10(4):689-99.

Colocalization of active KCa channels and Ca2+ channels within Ca2+ domains in helix neurons.

Author information

1
Laboratoire de Neurobiologie, CNRS 31, Marseille, France.

Abstract

Large conductance Ca(2+)-activated K+ channels (KCa channels) have to be colocalized with Ca2+ channels to be involved in nerve cell firing regulation. KCa channels were detected in cell-attached patches performed in voltage-clamped Helix neurons. This technique allowed Ca2+ entry to occur either within or around the patch. We observed that the submembrane Ca2+ diffusion was sufficiently limited to prevent the KCa channels from being opened by remote Ca2+ entry. The KCa channels located in areas devoid of Ca2+ channels remained quiescent during cell firing. These crypto channels were detected following either patch excision or intracellular Ca2+ injection. These data provide direct evidence for the existence of functional Ca2+ domains in a nonspecialized neural membrane.

PMID:
8386529
[Indexed for MEDLINE]

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