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Genes Dev. 1993 Apr;7(4):555-69.

The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit.

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  • 1Center for Molecular Medicine/Institute of Biotechnology, University of Texas Health Science Center, San Antonio 78245.

Abstract

The retinoblastoma protein (p110RB) interacts with many cellular proteins in complexes potentially important for its growth-suppressing function. We have developed and used an improved version of the yeast two-hybrid system to isolate human cDNAs encoding proteins able to bind p110RB. One clone encodes a novel type 1 protein phosphatase catalytic subunit (PP-1 alpha 2), which differs from the originally defined PP-1 alpha by an amino-terminal 11-amino-acid insert. In vitro-binding assays demonstrated that PP-1 alpha isoforms preferentially bind the hypophosphorylated form of p110RB. Moreover, similar p110RB sequences are required for binding PP-1 alpha 2 and SV40 large T antigen. Cell cycle synchrony experiments revealed that this association occurs from mitosis to early G1. The implications of these findings on the regulation of both proteins are discussed.

PMID:
8384581
[PubMed - indexed for MEDLINE]
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