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Vaccine. 1993;11(3):363-5.

A new method for rapidly removing contaminating micro-organisms from porcine parvovirus or pseudorabies virus master-seed suspensions.

Author information

1
National Veterinary Institute, Division of Vaccine Research, Uppsala, Sweden.

Abstract

Virus-contaminated cell cultures are a major problem in the bio-industry. Methods employed to date to remove contaminating micro-organisms are slow and costly, and a new method is proposed here which is simple and rapid. The method uses polyacrylamide beads coated with specific antibodies which yielded bead-antibody-virus complexes when suspended in the virus solution to be cleared. The purified virus was propagated in cells which show phagocytic activity. Vaccine master-seed virus is shown to be rapidly cleaned and propagated using this method.

PMID:
8383386
DOI:
10.1016/0264-410x(93)90200-h
[Indexed for MEDLINE]

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