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Gene. 1993 Feb 14;124(1):131-2.

Versatile gene cassette plasmids to facilitate the construction of generalized and specialized cloning vectors.

Author information

1
Department of Biotechnology, Faculty of Science, Mahidol University Bangkok, Thailand.

Abstract

We have built a series of useful gene cassette plasmids to facilitate the construction of generalized and specialized cloning vectors. The gene cassettes consist of two promoter-less genes, cat and gus, a selectable marker gene (tet) and an IncP mob sequence. All of these genes in the cassette vectors are flanked by many unique restriction sites to facilitate their use in the construction of cloning vectors.

PMID:
8382651
DOI:
10.1016/0378-1119(93)90773-v
[Indexed for MEDLINE]

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