Format

Send to

Choose Destination
Biochem J. 1993 Feb 15;290 ( Pt 1):179-83.

Organization of the phosphoinositide cycle. Assessment of inositol transferase activity in purified plasma membranes.

Author information

1
Department of Physiology & Biophysics, N.Y.U. Medical Center, New York.

Abstract

Experiments were carried out to determine whether or not CDP-diacylglycerol:myo-inositol 3-phosphatidyltransferase (IT) activity (EC 2.7.8.11) could be detected in purified plasma-membrane fractions from WRK-1 rat mammary tumour cells. These cells have previously been shown to have a very active phosphoinositide cycle. Sucrose-density-gradient-purified plasma membranes contained no IT activity that could not be accounted for by endoplasmic-reticulum contamination. However, we also determined that the relative amount of IT activity in endoplasmic reticulum and plasma-membrane fractions could be altered by changing the concentration of detergent in the assay system.

PMID:
8382477
PMCID:
PMC1132399
DOI:
10.1042/bj2900179
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center