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Eur J Pharmacol. 1993 Jan 4;244(1):99-101.

Neurotensin stimulates cyclic AMP formation in CHO-rNTR-10 cells expressing the cloned rat neurotensin receptor.

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Department of Psychiatry and Psychology, Mayo Foundation, Jacksonville, FL 32224.


Recently, we reported that the rat neurotensin receptor expressed in transfected Chinese hamster ovary cells (CHO-rNTR-10) mediates the hydrolysis of polyphosphoinositides. In this study, we show that neurotensin stimulated cyclic AMP formation in CHO-rNTR-10 cells in a dose-dependent manner (EC50 = 34 nM). Neurotensin also stimulated cyclic AMP formation in membranal preparations under Ca(2+)-free conditions. These results suggest that this cyclic AMP formation occurs from direct coupling of the neurotensin receptor to a type of GTP binding protein, mediating synthesis of cyclic AMP, and not from an increase in intracellular Ca2+ concentration from polyphosphoinositide hydrolysis.

[Indexed for MEDLINE]

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