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Biotechniques. 1993 Aug;15(2):260-2.

A rapid optimized protocol for downward alkaline Southern blotting of DNA.

Author information

1
Institute for Genetics, University of Cologne, FRG.

Abstract

A simple and efficient 2.5-h Southern blotting procedure is described that uses 0.4 M NaOH to transfer DNA in a downward direction. The resulting blots give signals that are both sharper and 30% stronger than those obtained by conventional upward-transferred blots.

PMID:
8373591
[Indexed for MEDLINE]

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