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J Cell Biochem. 1993 Jun;52(2):153-8.

ADP-ribosylation of nucleolar proteins in HeLa tumor cells.

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1
Institute of Tumorbiology-Cancer Research, University of Vienna, Austria.

Abstract

ADP-ribosylation reactions in nucleoli of exponentially growing HeLa cells were studied. Isolated nuclei or nucleoli were labeled with 32P-NAD; then the nucleolar proteins were analyzed by 1-dimensional and 2-dimensional polyacrylamide gel electrophoresis (PAGE) and modified proteins were detected by autoradiography. The labeled nucleolar proteins were also chromatographically fractionated on DEAE-cellulose. Electrophoretic analysis of total nucleolar and chromatographically purified proteins revealed that besides nuclear ADP-ribosyltransferase and histones two characteristic nucleolar phosphoproteins numatrin/B23 and nucleolin/C23 were modified by ADP-ribosylation.

PMID:
8366133
DOI:
10.1002/jcb.240520207
[Indexed for MEDLINE]

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