Format

Send to

Choose Destination
See comment in PubMed Commons below
Biochem Biophys Res Commun. 1993 Aug 31;195(1):58-64.

Protein identification by mass profile fingerprinting.

Author information

1
Department of Biology, Swiss Federal Institute of Technology (E.T.H.), Z├╝rich.

Abstract

We have developed an algorithm for identifying proteins at the sub-microgram level without sequence determination by chemical degradation. The protein, usually isolated by one- or two-dimensional gel electrophoresis, is digested by enzymatic or chemical means and the masses of the resulting peptides are determined by mass spectrometry. The resulting mass profile, i.e., the list of the molecular masses of peptides produced by the digestion, serves as a fingerprint which uniquely defines a particular protein. This fingerprint may be used to search the database of known sequences to find proteins with a similar profile. If the protein is not yet sequenced the profile can serve as a unique marker. This provides a rapid and sensitive link between genomic sequences and 2D gel electrophoresis mapping of cellular proteins.

PMID:
8363627
DOI:
10.1006/bbrc.1993.2009
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center