Format

Send to

Choose Destination
Plasmid. 1993 May;29(3):233-5.

Clostridium perfringens-Escherichia coli shuttle vectors that carry single antibiotic resistance determinants.

Author information

1
Department of Microbiology, Monash University, Clayton, Australia.

Abstract

Two versatile Clostridium perfringens-Escherichia coli shuttle vectors were constructed. Each plasmid carried a single antibiotic resistance gene which was expressed in both organisms. The plasmid pJIR750 encoded resistance to chloramphenicol and pJIR751 encoded resistance to erythromycin. Each plasmid contained the pUC18-derived multiple cloning site and the lacZ' gene which enabled direct screening for recombinants in E. coli. These plasmids should prove invaluable for the genetic manipulation of C. perfringens.

PMID:
8356117
DOI:
10.1006/plas.1993.1025
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center