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Nature. 1993 Aug 26;364(6440):809-14.

Emptying of intracellular Ca2+ stores releases a novel small messenger that stimulates Ca2+ influx.

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1
Department of Pharmacology, University of California, San Diego, La Jolla 92093-0647.

Abstract

Intracellular Ca2+ signals that last more than a few minutes after the onset of stimulation depend critically on influx of extracellular Ca2+. Such Ca2+ influx can be triggered in many cell types by depletion of intracellular Ca2+ stores without detectable elevations of known messengers. The mechanism by which store depletion can control plasma membrane Ca2+ permeability remains controversial. Here we present evidence for a novel soluble mediator. Calcium depletion of a lymphocyte cell line caused the messenger to be released from intracellular organelles into the cytoplasm and to a much lesser extent into the extracellular medium. The messenger caused Ca2+ influx when applied to macrophages, astrocytoma cells, and fibroblasts and was therefore named CIF (for Ca(2+)-influx factor). CIF appears to have hydroxyls (or hydroxyl and amino groups) on adjacent carbons, a phosphate, and a M(r) under 500.

Comment in

PMID:
8355806
DOI:
10.1038/364809a0
[Indexed for MEDLINE]

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