Abstract
A 0.4 kb polymerase chain reaction (PCR) product obtained from cDNA made from the midgut and Malpighian tubules of fifth instar larvae of Heliothis virescens was used to screen a larval midgut and Malpighian tubules cDNA library. Four clones were obtained, one of 1.9 kb and others of 1.4 kb. The 1.9 kb clone encodes a 17.2 kDa protein which is highly homologous to other vacuolar ATPases proteolipids. Putative N-glycosylation and DCCD binding sites were observed at amino acid residues 83 and 139, respectively.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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Digestive System / enzymology
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Larva / enzymology
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Malpighian Tubules / enzymology
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Molecular Sequence Data
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Moths / enzymology*
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Moths / genetics
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Proteolipids / chemistry*
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Proteolipids / genetics
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Proton-Translocating ATPases / chemistry*
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Proton-Translocating ATPases / genetics
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Sequence Homology, Amino Acid
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Vacuoles / enzymology
Substances
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Proteolipids
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Proton-Translocating ATPases