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FEBS Lett. 1993 Aug 9;328(1-2):125-9.

Mutation-spectrum of a true abasic site in codon 12 of a c-Ha-ras gene in mammalian cells.

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Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.


An abasic site is postulated to be a premutagenic lesion. We previously reported that a c-Ha-ras gene with an abasic site analogue at either the first or the second position of codon 12 induced the formation of foci by point-mutational activation of the gene [Kamiya et al. (1992) Nucleic Acids Res. 20, 4409-4415]. In this study we constructed c-Ha-ras genes with a true abasic site in codon 12. The ras genes were found to be activated in NIH3T3 cells by a mutation to A at the modified and flanking positions, and the flanking mutations were detected more frequently.

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