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Exp Cell Res. 1993 Aug;207(2):220-5.

Electron microscopic localization of ribosomal DNA in rat liver nucleoli by nonisotopic in situ hybridization.

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Departamento de Biología, Facultad de Ciencias, U.N.A.M., México D. F.


We have used postembedding nonisotopic in situ hybridization, with biotinylated rat ribosomal DNA (rDNA) as a probe and streptavidin coupled to 10-nm colloidal gold particles as the detection system, to localize rDNA sequences in rat liver nucleoli at the electron microscopic level. For comparison purposes, immunoelectron microscopy was performed for the detection of DNA. Our results indicate that ribosomal DNA sequences are enriched in the dense fibrillar component of the rat liver nucleolus. These data are discussed in relation to the putative site(s) for transcription of ribosomal genes.

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