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Nucleic Acids Res. 1993 Jul 11;21(14):3281-6.

Isolation, sequencing and overexpression of the gene encoding the theta subunit of DNA polymerase III holoenzyme.

Author information

1
University of Colorado Health Sciences Center, Department of Biochemistry, Biophysics and Genetics, Denver 80262.

Abstract

The gene encoding the theta subunit of DNA polymerase III holoenzyme, designated holE, was isolated using a strategy in which peptide sequence was used to derive a DNA hybridization probe. Sequencing of the gene, which maps to 41.43 centisomes of the chromosome, revealed a 76-codon open reading frame predicted to produce a protein of 8,846 Da. When placed in a tac promoter expression vector, the open reading frame directed expression of a protein, that comigrated with authentic theta subunit from purified holoenzyme, to 6% of total soluble protein.

PMID:
8341603
PMCID:
PMC309768
[Indexed for MEDLINE]
Free PMC Article

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